Cell selection from a murine tumour using the fluorescent probe Hoechst 33342

Cell Cycle Specific Toxicity of the Hoechst 33342 Stain in Untreated or Irradiated Murine Tumor Cells1

Hoechst 33342 stain has been used previously in conjunction with fluorescence activated cell sorting to separate cell populations into the various phases of the cell cycle. The present experiments were designed to evaluate the toxicity of this stain in cells derived from solid kill sarcomas which were untreated or irradiated prior to dissociation. In both untreated and irradiated cells, stain t...

Cell cycle specific toxicity of the Hoechst 33342 stain in untreated or irradiated murine tumor cells.

Hoechst 33342 stain has been used previously in conjunction with fluorescence activated cell sorting to separate cell populations into the various phases of the cell cycle. The present experiments were designed to evaluate the toxicity of this stain in cells derived from solid KHT sarcomas which were untreated or irradiated prior to dissociation. In both untreated and irradiated cells, stain to...

Effects of Hoechst 33342 on C2C12 and PC12 cell differentiation.

Accumulative evidence demonstrates that normal as well as cancer stem cells can be identified as a side population following Hoechst 33342 staining and flow cytometric analysis. This popular method is based on the ability of stem cells to efflux this fluorescent vital dye. We demonstrate that Hoechst 33342 can affect cell differentiation, suggesting potential complications in the interpretation...

Labeling nuclear DNA with hoechst 33342.

A number of fluorescent stains are available that label DNA and allow easy visualization of the nucleus in interphase cells and chromosomes in mitotic cells. One advantage of Hoechst 33342 is that it is membrane permeant and, thus, can stain live cells. Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. On binding to DNA, the fluorescence greatly increases. This pro...

Identification of a Cancer Stem Cell Enriched Side Population Using Hoechst 33342 Based Isolation